Welker N E, Campbell L L
J Bacteriol. 1967 Oct;94(4):1124-30. doi: 10.1128/jb.94.4.1124-1130.1967.
Eight strains of highly amylolytic, sporeforming bacilli (hereafter referred to as Bacillus amyloliquefaciens) were compared with respect to their taxonomic relationship to B. subtilis. The physiological-biochemical properties of these two groups of organisms showed that B. amyloliquefaciens differed from B. subtilis by their ability to grow in 10% NaCl, characteristic growth on potato plugs, increased production of alpha-amylase, and their ability to ferment lactose with the production of acid. The base compositions of the deoxyribonucleic acid (DNA) of the B. subtilis strains consistently fell in the range of 41.5 to 43.5% guanine + cytosine (G + C), whereas that of the B. amyloliquefaciens strains was in the 43.5 to 44.9% G + C range. Hybrid formation between B. subtilis W23 and B. amyloliquefaciens F DNA revealed only a 14.7 to 15.4% DNA homology between the two species. Transducing phage, SP-10, was able to propagate on B. subtilis W23 and B. amyloliquefaciens N, and would transduce B. subtilis 168 (indole(-)) and B. amyloliquefaciens N-10 (arginine(-)) to prototrophy with a frequency of 3.9 x 10(-4) and 2.4 x 10(-5) transductants per plaque-forming unit, respectively. Attempts to transduce between the two species were unsuccessful. These data show that Bacillus amyloliquefaciens is a valid species and should not be classified as a strain or variety of B. subtilis.
对八株高度解淀粉、产芽孢杆菌（以下简称解淀粉芽孢杆菌）与枯草芽孢杆菌的分类关系进行了比较。这两组生物体的生理生化特性表明，解淀粉芽孢杆菌与枯草芽孢杆菌的不同之处在于，它能在10%氯化钠中生长、在马铃薯块上有特征性生长、α-淀粉酶产量增加，以及能够发酵乳糖并产酸。枯草芽孢杆菌菌株的脱氧核糖核酸（DNA）碱基组成始终在鸟嘌呤+胞嘧啶（G + C）含量为41.5%至43.5%的范围内，而解淀粉芽孢杆菌菌株的G + C含量在43.5%至44.9%的范围内。枯草芽孢杆菌W23与解淀粉芽孢杆菌F DNA之间的杂交形成显示，这两个物种之间的DNA同源性仅为14.7%至15.4%。转导噬菌体SP-10能够在枯草芽孢杆菌W23和解淀粉芽孢杆菌N上繁殖，并能将枯草芽孢杆菌168（吲哚（-））和解淀粉芽孢杆菌N-10（精氨酸（-））转导为原养型，转导频率分别为每噬菌斑形成单位3.9×10⁻⁴和2.4×10⁻⁵个转导子。在这两个物种之间进行转导的尝试未成功。这些数据表明，解淀粉芽孢杆菌是一个有效的物种，不应被归类为枯草芽孢杆菌的一个菌株或变种。
