Département de phytologie, Faculté des sciences de l'agriculture et de l'alimentation, Université Laval, G1K 7P4, Sainte-Foy, Québec, Canada.
Planta. 1991 May;184(2):196-208. doi: 10.1007/BF00197948.
The accumulation of hydroxyproline-rich glycoproteins (HRGPs) in cell walls of dicotyledonous plants is thought to be involved in the defense response to pathogens. An antiserum raised against deglycosylated HRGPs from melon was used for studying the subcellular localization of these glycoproteins in susceptible and resistant tomato (Lycopersicon esculentum Mill.) root tissues infected by Fusarium oxysporum f.sp. radicis-lycopersici. A time-course of HRGP accumulation revealed that these glycoproteins increased earlier and to a higher extent in resistant than in susceptible cultivars. In the compatible interaction, increase in HRGPs was largely correlated with pathogen invasion and appeared to occur as a result of wall damage. In the incompatible interaction, HRGPs accumulated in the walls of uninvaded cells, thus indicating a possible role in the protection against fungal penetration. The occurrence of substantial amounts of HRGPs in papillae, known to be physical barriers formed in response to infection, and in intercellular spaces provides additional support to the concept that such glycoproteins play an important role in disease resistance.
细胞壁中富含羟脯氨酸的糖蛋白（HRGPs）的积累被认为与双子叶植物对病原体的防御反应有关。针对甜瓜去糖基化 HRGPs 制备的抗血清被用于研究易感和抗病番茄（Lycopersicon esculentum Mill.）根组织在感染尖孢镰刀菌（Fusarium oxysporum f.sp. radicis-lycopersici）时这些糖蛋白的亚细胞定位。HRGP 积累的时间进程表明，这些糖蛋白在抗病品种中的积累比在感病品种中更早、更显著。在亲和互作中，HRGPs 的增加与病原体的入侵密切相关，似乎是由于细胞壁受损所致。在不亲和互作中，HRGPs 在未受侵染的细胞壁中积累，因此表明其可能在抵御真菌穿透中发挥作用。在已知作为对感染的反应而形成的物理屏障——乳突和细胞间隙中存在大量 HRGPs，这为这些糖蛋白在抗病性中发挥重要作用的观点提供了额外的支持。
