Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11 000, Belgrade, Yugoslavia.
Planta. 1991 May;184(2):248-53. doi: 10.1007/BF00197954.
We devised recently a method to trap intact isolated chloroplasts on a solid support consisting of membrane filters made of cellulose nitrate (Cerović et al., 1987, Plant Physiol. 84, 1249-1251). The addition of alkaline phosphatase to the reaction medium enabled continuous photosynthesis by spinach (Spinacia oleracea L.) chloroplasts to be sustained by hydrolysis of newly produced and exported triose phosphates and recycling of orthophosphate. In this system, simultaneous measurements of chlorophyll fluorescence and oxygen evolution were performed and their dependence on orthophosphate concentration was investigated. Optimal photosynthesis was obtained at a much higher initial orthophosphate concentration (2-4 mM) compared to intact chloroplasts in suspension. Secondary kinetics of chlorophyll fluorescence yield were observed and were shown to depend on the initial orthophosphate concentration.
我们最近设计了一种方法,将完整的分离叶绿体困在由硝酸纤维素制成的膜滤器(纤维素硝酸酯)的固体支撑物上(Cerović 等人,1987 年,植物生理学 84, 1249-1251)。向反应介质中添加碱性磷酸酶,使菠菜(Spinacia oleracea L.)叶绿体通过新产生和输出的三碳磷酸的水解和正磷酸盐的循环,持续进行光合作用。在该系统中,同时进行叶绿素荧光和氧气释放的测量,并研究了它们对正磷酸盐浓度的依赖性。与悬浮的完整叶绿体相比,在初始正磷酸盐浓度(2-4 mM)高得多的情况下,获得了最佳光合作用。观察到叶绿素荧光产量的二次动力学,并表明其依赖于初始正磷酸盐浓度。