Laboratoire pour l'Utilisation du Rayonnement Electromagnetique, Université de Paris XI, 91405, Orsay, France.
Photosynth Res. 1993 Jun;36(3):193-204. doi: 10.1007/BF00033038.
A newly developed nitrogen laser fluorimeter insensitive to actinic illumination was used to follow simultaneously the light induced changes in red and blue fluorescence of intact isolated spinach chloroplasts and leaf pieces. The recorded variable blue fluorescence was linked to a water soluble component of intact isolated chloroplasts, depended on Photosystem I, and was related to changes in carbon metabolism. From the comparison of changes in intact and broken chloroplasts and from fluorescence spectra under different conditions, it was concluded that the variation in NADPH was the major cause for the changes in blue fluorescence. This study opens a path towards continuous and non-destructive monitoring of NADPH redox state in chloroplasts and leaves.
一种新开发的对光解光不敏感的氮激光荧光计被用于同时跟踪完整的分离菠菜叶绿体和叶片的红光和蓝光荧光的光诱导变化。记录的可变蓝光荧光与完整的分离叶绿体中的一种水溶性成分有关，依赖于光系统 I，并且与碳代谢的变化有关。从完整和破碎叶绿体的变化比较以及在不同条件下的荧光光谱来看，可以得出结论，NADPH 的变化是导致蓝光荧光变化的主要原因。这项研究为连续和非破坏性监测叶绿体和叶片中的 NADPH 氧化还原状态开辟了道路。
