Research Institute for Photosynthesis, University of Sheffield, S10 2TN, Sheffield, UK.
Photosynth Res. 1987 Jan;11(2):119-30. doi: 10.1007/BF00018270.
Simultaneous "ripples" (sudden changes in rate) in CO2 dependent O2 evolution and associated chlorophyll a fluorescence were followed in isolated, largely intact, spinach chloroplasts. These ripples could only be observed under conditions in which the supply of inorganic phosphate was limiting. This limitation was achieved either by 1) omission of phosphate in the assay medium, 2) use of inhibitors of the phosphate translocator, or 3) the addition of triose phosphate, a competitive inhibitor of Pi for the same translocator.The possible relation of these ripples to the dampening oscillations that can be observed in leaves, leaf pieces, isolated cells and protoplasts, is discussed.
在分离的、基本完整的菠菜叶绿体中，观察到 CO2 依赖的 O2 演化和相关叶绿素 a 荧光的同时“涟漪”（速率的突然变化）。这些涟漪只能在无机磷酸盐供应有限的条件下观察到。这种限制可以通过以下方式实现：1）在测定介质中省略磷酸盐，2）使用磷酸盐转运体抑制剂，或 3）添加三磷酸糖，这是磷酸盐转运体的竞争性抑制剂。讨论了这些涟漪与可以在叶片、叶片碎片、分离细胞和原生质体中观察到的阻尼振荡之间的可能关系。
