用于评估猪左心室心肌瘢痕的三维相敏反转恢复快速扰相梯度回波序列
Three-dimensional phase-sensitive inversion-recovery Turbo FLASH sequence for the assessment of left ventricular myocardial scar in swine.
作者信息
Chen Xiuyu, Lu Minjie, Yin Gang, Zhao Tao, Shao Xiaoning, Zhao Ranxu, Tang Yue, An Jing, Jiang Shiliang, Zhao Shihua
机构信息
Department of Radiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
出版信息
PLoS One. 2013 Oct 23;8(10):e78305. doi: 10.1371/journal.pone.0078305. eCollection 2013.
OBJECTIVES
We sought to evaluate the feasibility and accuracy of free-breathing three-dimensional (3D) phase-sensitive inversion-recovery (PSIR) Turbo FLASH sequence for noninvasive assessment of left ventricular myocardial scar in swine models.
MATERIALS AND METHODS
Nine Chinese minipigs with experimentally induced acute myocardial infarction were studied. At 1 week and the study endpoint 4 weeks after myocardial infarction surgery, the 3D and 2D contrasted cardiac magnetic resonance (CMR) imaging were performed randomly by using a 1.5 T clinical MR imaging system. Comparisons of myocardial scar volume (in cubic centimeters), scar transmurality (on a 5 points scale) and image quality (on a 4 points Likert scale) were performed by using the Pearson correlation and Bland-Altman analysis (for myocardial scar volume) or κ statistics (for transmurality) or Wilcoxon signed rank test (for image quality).
RESULTS
In 6 of the 9 pigs, all procedures were successfully completed. In these pigs, a total of 48 segments with myocardial scars were detected by both 3D and 2D sequences, and there was good agreement for classification of scar transmurality (κ=0.930). The scar volume determined by triphenyltetrazolium chloride (TTC) staining (3.52 ± 1.40 cm(3)) showed a good correlation with both 3D (3.54 ± 1.36 cm(3), r=0.957, P=0.003) and 2D sequence (3.53 ± 1.26 cm(3), r=0.942, P=0.005) at 4 weeks. And there were good correlation between scar volumes obtained from 3D and 2D techniques (r=0.859, P<0.001) at both time points. Both 3D and 2D images detected a small reduction of scar volume from week 1 to week 4 by a factor of 1.179 and 1.176, respectively. Although slightly more artifacts were observed on 2D PSIR images, the overall image quality was not significantly different between the two sequences (3.17 ± 0.83 for 2D vs. 3.25 ± 0.75 for 3D, P =0.655).
CONCLUSIONS
The free-breathing 3D PSIR Turbo FLASH sequence enables accurate assessment of left ventricular myocardial scar.
目的
我们旨在评估自由呼吸三维（3D）相敏反转恢复（PSIR）快速成像序列在猪模型中无创评估左心室心肌瘢痕的可行性和准确性。
材料与方法
对9只实验诱导急性心肌梗死的中国小型猪进行研究。在心肌梗死手术后1周和4周的研究终点，使用1.5T临床磁共振成像系统随机进行三维和二维对比心脏磁共振（CMR）成像。采用Pearson相关性分析和Bland-Altman分析（用于心肌瘢痕体积）或κ统计（用于透壁性）或Wilcoxon符号秩检验（用于图像质量）对心肌瘢痕体积（立方厘米）、瘢痕透壁性（5分制）和图像质量（4分Likert量表）进行比较。
结果
9只猪中的6只成功完成了所有检查。在这些猪中，三维和二维序列均检测到共48个有心肌瘢痕的节段，瘢痕透壁性分类具有良好的一致性（κ=0.930）。在4周时，通过氯化三苯基四氮唑（TTC）染色测定的瘢痕体积（3.52±1.40cm³）与三维（3.54±1.36cm³，r=0.957，P=0.003）和二维序列（3.53±1.26cm³，r=0.942，P=0.005）均具有良好的相关性。在两个时间点，三维和二维技术获得的瘢痕体积之间均具有良好的相关性（r=0.859，P<0.001）。从第1周到第4周，三维和二维图像均检测到瘢痕体积略有减小，分别减小了1.179倍和1.176倍。虽然在二维PSIR图像上观察到的伪影略多，但两个序列的整体图像质量无显著差异（二维为3.17±0.83，三维为3.25±0.75，P =0.655）。
结论
自由呼吸三维PSIR快速成像序列能够准确评估左心室心肌瘢痕。
Zotero 插件