Wheat Research Unit, Division of Plant Industry, CSIRO, P.O. Box 7, 2113, North Ryde, NSW, Australia.
Theor Appl Genet. 1993 Jan;85(5):616-24. doi: 10.1007/BF00220921.
A genomic fragment containing the Bx17 high-molecular-weight (HMW) glutenin gene was isolated from a wheat genomic library. The fragment contains a coding region of 2.82kb with 1.98-kb downstream and 12.8-kb upstream flanking regions. The fragment was sequenced and compared with previously published glutenin genes from chromosomes 1A, 1B and 1D using a computer alignment package. The Bx17 gene shows marked similarity to the Bx7 gene sequence. A phenetic tree derived from the alignments is presented. Also shown are restriction fragment length polymorphisms (RFLPs) at the glutenin loci in a set of Australian and international wheat varieties using different regions of the glutenin clone as probes. The RFLPs correlated well with the protein composition in all cultivars analysed.
从一个小麦基因组文库中分离出一个包含 Bx17 高分子量 (HMW) 麦谷蛋白基因的基因组片段。该片段包含一个 2.82kb 的编码区，下游有 1.98kb，上游有 12.8kb 的侧翼区。该片段经过测序，并使用计算机比对软件与之前发表的来自 1A、1B 和 1D 染色体的麦谷蛋白基因进行了比较。Bx17 基因与 Bx7 基因序列具有显著的相似性。本文还展示了利用麦谷蛋白克隆的不同区域作为探针，对一组澳大利亚和国际小麦品种的麦谷蛋白基因座的限制性片段长度多态性 (RFLP)。在所有分析的品种中，RFLP 与蛋白质组成相关性良好。
