鹰嘴豆（Cicer arietinum L.）愈伤组织体细胞胚胎发生和植株再生。

Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.).

机构信息

Department of Biosciences, University of Jammu, 180004, Jammu, India.

出版信息

Plant Cell Rep. 1993 Jul;12(9):521-4. doi: 10.1007/BF00236100.

PMID:24196114

Abstract

Somatic embryogenesis and plant regeneration were obtained from immature leaflet callus of chickpea. Numerous globular embryos developed on the surface of callus on Murashige and Skoog's (1962) medium containing 25 μM 2,4-dichlorophenoxyacetic acid. These globular embryos differentiated into mature somatic embryos upon removal of 2,4-dichlorophenoxyacetic acid. The maturation of embryos was significantly affected by pH, photoperiod, abscisic acid and genotype. Callus continued to produce somatic embryos for over 8 subcultures at 4 week intervals. Two per cent of the embryos formed plants on medium containing 15 μM gibberellic acid and 1 μM indole-3-butyric acid. Desiccation of embryos for a period of 3 d increased their rate of conversion into plants from 0.9 to 2.8%. All regenerated plants showed normal morphological characteristics.

摘要

从小扁豆未成熟的小叶愈伤组织中获得体细胞胚胎发生和植株再生。在含有 25μM 2,4-二氯苯氧乙酸的 Murashige 和 Skoog（1962）培养基上，愈伤组织表面形成了许多球形胚。去除 2,4-二氯苯氧乙酸后，这些球形胚分化成成熟的体细胞胚。胚的成熟受 pH 值、光周期、脱落酸和基因型的显著影响。每隔 4 周进行 8 次以上的继代培养，愈伤组织继续产生体细胞胚。在含有 15μM 赤霉素和 1μM 吲哚-3-丁酸的培养基上，2%的胚形成植株。将胚干燥 3 天可将其转化为植株的比率从 0.9 提高到 2.8%。所有再生植株均表现出正常的形态特征。