鹰嘴豆（Cicer arietinum L.）通过体细胞胚胎发生进行植株再生。

Plant regeneration via somatic embryogenesis in chick pea (Cicer arietinum L.).

机构信息

Division of Plant Tissue Culture, National Chemical Laboratory, 411008, Pune, India.

出版信息

Plant Cell Rep. 1993 Sep;12(11):652-5. doi: 10.1007/BF00232818.

PMID:24201882

Abstract

Five genotypes of chickpea (Cicer arietinum L.) PG1, PG5, PG12, N59 and C235 were evaluated for induction of somatic embryogenesis. Somatic embryogenesis was induced from immature cotyledons of genotypes PG12 and C235 and immature embryo axes of genotypes PG5, PG12 and C235. Genotypes N59 and PG1 showed no response. The maximum frequency of globular embryo formation occurred in cotyledonary segments on MS medium with 3.0 mg/l 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). Further embryo development was achieved only in somatic embryos derived from cotyledonary segments of genotype PG12. Globular-stage embryos derived from immature embryo axes of PG5, C235, PG12, and cotyledonary segments of C235 dedifferentiated and formed callus. The cotyledonary stage embryos of genotype PG12 germinated on half-strength MS medium supplemented with 1 mg/l zeatin. The regenerated plants were transferred to soil and grown to maturity.

摘要

五种鹰嘴豆（Cicer arietinum L.）PG1、PG5、PG12、N59 和 C235 基因型被评估用于诱导体细胞胚胎发生。体细胞胚胎发生从 PG12 和 C235 的未成熟子叶和 PG5、PG12 和 C235 的未成熟胚轴中诱导。基因型 N59 和 PG1 没有反应。在含有 3.0 mg/l 2,4,5-三氯苯氧乙酸（2,4,5-T）的 MS 培养基上，子叶切段中出现最大频率的球形胚形成。只有来自 PG12 基因型子叶切段的体细胞胚胎才能进一步发育。来自 PG5、C235、PG12 的未成熟胚轴和 C235 的子叶切段的球形期胚胎去分化并形成愈伤组织。基因型 PG12 的子叶期胚胎在添加 1 mg/l 玉米素的半强度 MS 培养基上发芽。再生植物被转移到土壤中并生长成熟。