Dutta Gupta S, Ahmed R, De D N
Department of Agricultural and Food Engineering, Indian Institute of Technology, 721302, Kharagpur, India.
Plant Cell Rep. 1997 Jun;16(9):628-631. doi: 10.1007/BF01275504.
Excised seedling leaf segments of winged bean [Psophocarpus tetragonolobus (L.) DC.] underwent direct somatic embryogenesis under appropriate incubation conditions. Initiation and development of the somatic embryos occurred using a two-step culture method. The culture procedure involved incubation for 28 days on MS basal medium supplemented with 0.1-0.5 mg/l NAA and 1.0-2.0 mg/l BA (induction medium) before transfer to MS medium supplemented with 0.1 mg/l IAA and 2.0 mg/l BA (embryo development medium). The initial exposure to low levels of NAA coincident with high levels of BA in the induction medium was essential for embryogenic induction. Maximum embryogenesis (43.3%) was obtained with 0.2 mg/l NAA and 2.0 mg/l BA, and at least 14 days on induction medium were required prior to transfer to the embryo development medium. The conversion frequency of cotyledonary embryos was 53.3% upon culture on MS medium containing 0.1 mg/l ABA for 7 days followed by transfer to MS medium supplemented with 0.1 mg/l IBA and 0.2 mg/l BA. Following conversion, the regenerated plantlets were transferred to soil and showed normal morphological characteristics.
在适宜的培养条件下，切除的四棱豆[Psophocarpus tetragonolobus (L.) DC.]幼苗叶片切段可进行直接体细胞胚胎发生。体细胞胚胎的起始和发育采用两步培养法。培养过程包括在添加了0.1 - 0.5毫克/升萘乙酸（NAA）和1.0 - 2.0毫克/升苄氨基腺嘌呤（BA）的MS基本培养基上培养28天（诱导培养基），然后转移到添加了0.1毫克/升吲哚乙酸（IAA）和2.0毫克/升BA的MS培养基上（胚胎发育培养基）。在诱导培养基中最初接触低水平的NAA并同时接触高水平的BA对胚胎发生诱导至关重要。使用0.2毫克/升NAA和2.0毫克/升BA时可获得最大胚胎发生率（43.3%），并且在转移到胚胎发育培养基之前，在诱导培养基上至少需要培养14天。子叶胚在含有0.1毫克/升脱落酸（ABA）的MS培养基上培养7天，然后转移到添加了0.1毫克/升吲哚丁酸（IBA）和0.2毫克/升BA的MS培养基上，其转化率为53.3%。转化后，再生的植株被转移到土壤中，并表现出正常的形态特征。
