Department of Animal Science, McGill University, Ste. Anne de Bellevue, H9X 3V9, Quebec, Canada.
Theor Appl Genet. 1993 Feb;85(8):931-6. doi: 10.1007/BF00215031.
Two strains of meat-type chickens which had been derived from the same genetic base, but were selected for high or low abdominal fat content, respectively, were analyzed for polymorphisms in the growth hormone gene (GH). A total of four DNA polymorphisms were identified, one at a SacI restriction site and three at MspI restriction sites. Restriction mapping indicated that all polymorphisms were in exons and/or introns and not in flanking regions of the gene. The incidence of GH polymorphisms was determined in 20 chickens from each strain and significant differences were observed for two of the four polymorphisms. Analysis by DNA fingerprinting using (CAC)5 as a probe indicated that the inbreeding coefficient was 0.1 in both strains and that random genetic drift was minimal. Thus, the selection for abdominal fat appears to have affected the frequency of alleles of the growth hormone gene. Whether this is the direct consequence of an altered growth hormone gene on fat metabolism or reflects linkage to an allele of a neighbouring gene remains to be determined.
两种肉用型鸡，它们源自相同的遗传基础，但分别选择了高或低的腹部脂肪含量，对其生长激素基因 (GH) 的多态性进行了分析。共发现了 4 种 DNA 多态性，其中 1 种位于 SacI 限制位点，3 种位于 MspI 限制位点。限制图谱表明，所有多态性都位于基因的外显子和/或内含子中，而不在侧翼区域。在每个品系的 20 只鸡中确定了 GH 多态性的发生率，观察到了 4 种多态性中的两种存在显著差异。使用 (CAC)5 作为探针的 DNA 指纹分析表明，两个品系的近交系数均为 0.1，随机遗传漂变最小。因此，腹部脂肪的选择似乎影响了生长激素基因等位基因的频率。这种情况是否是生长激素基因对脂肪代谢的直接影响，或者反映了与邻近基因的等位基因的连锁，还有待确定。
