Ali S, Müller C R, Epplen J T
Hum Genet. 1986 Nov;74(3):239-43. doi: 10.1007/BF00282541.
Interspersed simple repetitive DNA is a convenient genetic marker for analysis of restriction fragment length polymorphisms (RFLPs) because of the numbers and the frequencies of its alleles. Oligonucleotide probes specific for variations of the GATCA simple repeats have been designed and hybridized to a panel of human DNAs digested with various restriction enzymes. Numerous RFLPs were demonstrated in AluI and MboI digested DNA with "pure" GATA oligonucleotides as probes. The optimal length of the probe for RFLP analysis was 20 bases taking into account fragment lengths (1.5-7 kilobases = kb), signal to background ratio, and number of clearly evaluable RFLPs. By using different restriction enzymes individual-specific hybridization patterns ("DNA fingerprints") can be established. Hypervariable simple repeat fragments are stably inherited in a Mendelian fashion. Advantages of this method are discussed.
散布的简单重复DNA因其等位基因的数量和频率，是用于分析限制性片段长度多态性（RFLP）的便捷遗传标记。已设计出针对GATCA简单重复序列变异的寡核苷酸探针，并将其与用各种限制性酶消化的一组人类DNA进行杂交。以“纯”GATA寡核苷酸为探针，在经AluI和MboI消化的DNA中证实了大量RFLP。考虑到片段长度（1.5 - 7千碱基 = kb）、信号与背景比率以及可清晰评估的RFLP数量，用于RFLP分析的探针最佳长度为20个碱基。通过使用不同的限制性酶，可以建立个体特异性杂交模式（“DNA指纹”）。高变简单重复片段以孟德尔方式稳定遗传。本文讨论了该方法的优点。
