Feng X P, Kuhnlein U, Fairfull R W, Aggrey S E, Yao J, Zadworny D
Department of Animal Science, McGill University, Quebec, Canada.
J Hered. 1998 Jul-Aug;89(4):355-9. doi: 10.1093/jhered/89.4.355.
A genomic clone spanning 16 kb of the GH receptor gene was mapped and used as a probe for identifying restriction fragment length polymorphisms (RFLPs) in chickens. Several strains of meat-type and egg laying chickens were found to segregate for an HindIII RFLP located in the intron preceding exon 4. The polymorphic HindIII site overlapped with a poly(A) signal. Association of the HindIII RFLP with traits was analyzed in a random-bred White Leghorn strain in three generations using either selective or random genotyping. Both methods revealed significant association of the HindIII+ allele (presence of the poly(A) signal) with an increased juvenile body weight (130 days of age). In two meat-type strains divergently selected for size of the abdominal fat pad, the HindIII+ allele was coselected with leanness. The results indicate the presence of a genetic variant of the GH receptor gene which affects growth and abdominal fat deposition and which is relatively frequent in egg laying as well as in meat-type chickens.
绘制了一个跨越16 kb生长激素受体基因的基因组克隆图谱，并将其用作探针来鉴定鸡的限制性片段长度多态性（RFLP）。发现几种肉用型和蛋用型鸡品系在位于外显子4之前的内含子中的HindIII RFLP处发生分离。多态性的HindIII位点与一个聚腺苷酸化信号重叠。使用选择性或随机基因分型方法，在一个随机交配的白来航鸡品系中对三代鸡进行了HindIII RFLP与性状的关联分析。两种方法均显示HindIII +等位基因（存在聚腺苷酸化信号）与幼年体重增加（130日龄）显著相关。在两个针对腹部脂肪垫大小进行了不同选择的肉用型品系中，HindIII +等位基因与瘦肉率共同被选择。结果表明存在一种生长激素受体基因的遗传变异，它影响生长和腹部脂肪沉积，并且在蛋用型和肉用型鸡中相对常见。
