花梗芽茎尖培养繁殖蝴蝶兰和文心兰。

Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds.

机构信息

Research and Development Center, Orchid Sanctuary Dogashima, Nishina, Nishiizu-cho, Kamo-gun, 410-35, Shizuoka, Japan.

出版信息

Plant Cell Rep. 1993 Nov;13(1):7-11. doi: 10.1007/BF00232306.

PMID:24196174

Abstract

Green Protocorm-like Bodies (PLB) with high multiplication capacity were induced from shoot tips of flower stalk buds having 1 or 2 leaf primordia using New Dogashima Medium (NDM) containing 0.1 mg l(-1) α-naphthaleneacetic acid (NAA) and 1 mg 1(-1) 6-benzylaminopurine (BAP). These PLB were subcultured on the same medium. More than 10,000 PLBs were obtained from a few buds on a single flower stalk within one year. After transfer onto NDM containing no plant growth regulator (PGR), the PLB developed into plantlets. The micropropagation method formulated in this study was applicable to 12 different genotypes. These results suggest that the methodology could be used on a commercial scale for vegetative propagation of Phalaenopsis and Doritaenopsis.

摘要

从具有 1 或 2 个叶原基的花梗芽的茎尖诱导出具有高增殖能力的绿色原球茎样体（PLB），使用含有 0.1 mg l(-1)α-萘乙酸（NAA）和 1 mg l(-1)6-苄基氨基嘌呤（BAP）的新狗岛培养基（NDM）。这些 PLB 在相同的培养基上进行继代培养。在一年内，从单个花梗上的几个芽中获得了超过 10000 个 PLB。将 PLB 转移到不含植物生长调节剂（PGR）的 NDM 上后，PLB 发育成小植株。本研究制定的微繁殖方法适用于 12 种不同基因型。这些结果表明，该方法可以在商业规模上用于蝴蝶兰和兜兰的营养繁殖。