兰花属离体多倍体诱导的最优方案

Optimal protocol for in vitro polyploid induction of Cymbidium aloifolium (L.) Sw.

机构信息

Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.

Salt Tolerant Rice Research Group, Khon Kaen University, Khon Kaen, 40002, Thailand.

出版信息

BMC Plant Biol. 2023 Jun 2;23(1):295. doi: 10.1186/s12870-023-04314-8.

DOI:10.1186/s12870-023-04314-8

PMID:37264309

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10236726/

Abstract

BACKGROUND

Cymbidium aloifolium is a popular ornamental flower in Thailand with both economic and medical values. Polyploid induction techniques are used to improve plant quality. This study identified polyploidy levels of C. aloifolium induction by colchicine. Protocorms of C. aloifolium were treated on solid New Dogashima Medium (NDM) with various concentrations of colchicine (0, 0.01, 0.02, 0.03, 0.04 and 0.05% w/v) for 2, 4 and 8 weeks.

RESULTS

Colchicine effectively induced plant polyploidy. Tetraploid plants were found after treatment with 0.03% and 0.04% colchicine for 8 weeks, while at increased concentration and duration, survival, response and growth performance decreased. Tetraploid plants showed the lowest growth performance but highest size of stomatal and epidermal cells. Growth performance and leaf surface anatomy data were analyzed by Pearson's correlation and PCA. Results showed that stomatal and epidermal cell sizes had strongly negative correlations with other variables, while HCA revealed that stomatal and epidermal cell sizes of tetraploid plantlets were larger but stomatal and epidermal cell densities decreased when compared with the diploids.

CONCLUSION

Colchicine at suitable concentrations and duration produced polyploid plants with alteration of morphological and anatomical traits. This study provides potential information to support orchid quality production as ornamental plants and a source of pharmaceutical raw materials.

摘要

背景

蝴蝶兰是泰国一种具有经济和药用价值的流行观赏花卉。多倍体诱导技术用于改善植物品质。本研究通过秋水仙素鉴定了蝴蝶兰的多倍体诱导水平。将蝴蝶兰原球茎在含有不同浓度秋水仙素（0、0.01、0.02、0.03、0.04 和 0.05%w/v）的固体新狗岛培养基（NDM）上处理 2、4 和 8 周。

结果

秋水仙素能有效诱导植物多倍体。用 0.03%和 0.04%的秋水仙素处理 8 周后发现了四倍体植株，而随着浓度和时间的增加，存活率、反应和生长性能下降。四倍体植株表现出最低的生长性能，但气孔和表皮细胞的大小最高。通过 Pearson 相关分析和 PCA 对生长性能和叶片表面解剖数据进行了分析。结果表明，气孔和表皮细胞大小与其他变量呈强烈负相关，而 HCA 表明，与二倍体相比，四倍体植株的气孔和表皮细胞较大，但气孔和表皮细胞密度降低。