School of Life Sciences, JNU, 110067, New Delhi, India.
Plant Cell Rep. 1994 Aug;13(11):623-7. doi: 10.1007/BF00232933.
Plant regeneration, which is the major limiting factor for transformation of Cajanus cajan, has been obtained via multiple shoot formation from the cotyledonary node region of seedlings germinated on MS medium containing 2 mgl(-1) 6-benzylaminopurine. A mass of multiple shoot-initials formed at the axillary bud region of the cotyledonary node of the seedlings within two weeks. The cotyledonary node along with the mass of shoot-initials excised from the seedling, continued to form new shoot-initials on MS medium containing 6-benzylaminopurine (2 mgl(-1)) and supplemented topically with indole-3-acetic acid. The formation of new shoot-initials was also observed from the cotyledonary nodal explant, after cutting off its surface layers to completely remove the pre-existing shoot-initials and culturing it on 6-benzylaminopurine (2 mgl(-1)) containing medium. The shoots elongated rapidly on basal MS medium and rooted efficiently in MS medium supplemented with indole-3-butyric acid (0.5 mgl(-1)). The procedure described is efficient, and highly reproducible and a common response was observed for all the six varieties tested.
利用含有 2mg/L6-苄基腺嘌呤的 MS 培养基对萌发的幼苗子叶节点区域进行丛生芽诱导，实现了兵豆的植株再生，这是兵豆转化的主要限制因素。在两周内，幼苗子叶节点的腋芽区域形成了大量丛生芽原基。从幼苗上切下子叶节点连同丛生芽原基，继续在含有 6-苄基腺嘌呤（2mg/L）的 MS 培养基上形成新的丛生芽原基，并局部补充吲哚-3-乙酸。在完全去除原有丛生芽原基并在含有 6-苄基腺嘌呤（2mg/L）的培养基上培养后，也可以从子叶节点外植体上观察到新丛生芽原基的形成。在基础 MS 培养基上，芽迅速伸长，并在添加吲哚-3-丁酸（0.5mg/L）的 MS 培养基上有效生根。所描述的方法效率高，重现性好，对所有六种测试品种都观察到了相同的反应。
