Texas Agricultural Experiment Station, Texas A&M University Research and Extension Center, 17360 Coit Rd., 75252-6599, Dallas, Texas, USA.
Plant Cell Rep. 1993 Dec;13(2):115-8. doi: 10.1007/BF00235303.
Hypocotyl explants of Albizzia julibrissin were placed on Gamborg's B5 medium supplemented with various levels of paclobutrazol, uniconazole, prohexadione calcium, or GA3. Callus formation was evident within one week after placement of the explants on the culture media. Green nodule-like structures protruded from the distal end of the explants within 10 days and developed into shoots within a month. These shoots readily formed adventitious roots when placed on fresh culture medium. All three of the gibberellin biosynthesis inhibitors increased shoot formation compared to the control. The number of shoots per explants was increased 107, 79, and 168% by 0.3-0.4 μM paclobutrazol, uniconazole, and prohexadione calcium, respectively. In contrast to the gibberellin biosynthesis inhibitors, GA3 decreased shoot formation. These results indicate that modification of gibberellin status can have a strong impact on the number of shoots formed.
金合欢胚轴外植体被放置在添加了不同浓度多效唑、烯效唑、调环酸钙或 GA3 的 Gamborg's B5 培养基上。外植体放在培养基上一周内即可观察到愈伤组织的形成。10 天内，绿色的结节状结构从外植体的远端突出，并在一个月内发育成芽。这些芽在新鲜培养基上很容易形成不定根。与对照相比，三种赤霉素生物合成抑制剂都增加了芽的形成。0.3-0.4 μM 多效唑、烯效唑和调环酸钙分别使每个外植体上的芽数增加了 107%、79%和 168%。与赤霉素生物合成抑制剂相反，GA3 减少了芽的形成。这些结果表明，赤霉素状态的改变对芽的形成数量有很大的影响。
