Department of Plant Cytology and Cytochemistry, University of Lodz, 90-237, 12/16, Lodz, Banacha, Poland.
Theor Appl Genet. 1992 Nov;85(2-3):303-8. doi: 10.1007/BF00222874.
A genomic DNA library of Lupinus luteus cv. Ventus was constructed in the phage vector EMBL3 using Mb oI-digested DNA. Screening with a 1070 bp labelled repetitive unit from L. luteus yielded several DNA clones. The repetitive family is composed of elements whose length is at least 16 kb. The average copy number of the cloned fragments is 5.0 × 10(4) per haploid genome and constitutes approximately 3% of the total L. luteus genome. The homologous repeats were found in all ten cultivars of L. luteus tested but were not detected in two cultivars each of the closely related species Lupinus albus and Lupinus angustifolius. The EcoRI family fragments could thus be considered as species-specific DNA elements. These fragments may be useful as molecular markers in the genetic manipulation of L. luteus.
利用 Mb oI 消化的 DNA，在噬菌体载体 EMBL3 中构建了 Lupinus luteus cv. Ventus 的基因组 DNA 文库。用来自 L. luteus 的 1070bp 标记重复单元进行筛选，得到了几个 DNA 克隆。该重复家族由长度至少为 16kb 的元件组成。克隆片段的平均拷贝数为每个单倍体基因组 5.0×10(4)，约占 L. luteus 基因组的 3%。在测试的 10 个 Lupinus luteus 品种中都发现了同源重复序列，但在两个密切相关的种 Lupinus albus 和 Lupinus angustifolius 的每个品种中都没有检测到。因此，EcoRI 家族片段可以被认为是种特异性 DNA 元件。这些片段可用作 Lupinus luteus 遗传操作的分子标记。
