Vollmer G, Layer P G
Neurosci Lett. 1986 Jan 2;63(1):91-5. doi: 10.1016/0304-3940(86)90019-4.
We report here a striking age dependency for tissue reconstruction capacity in rotating cultures of chick retinal cells, in particular in mixtures of retinal and pigment epithelial cells. The comparison of sections after acetylcholinesterase histochemistry delivered the following results. In aggregates from retinal cells, sorting out is clearly observable, but only a minor age-dependent effect is detectable, e.g. the laminar order of aggregates derived from 6-day-old cells is increased compared to aggregates from other stages. In aggregates from mixtures of retinal and pigment epithelial cells, the degree of laminar order is highest with cells from 5-day-old embryos and decreases significantly at older ages. The highest number of aggregates with the reconstruction of all main retinal layers was obtained by the reaggregation of retinal cells of embryonic day 5 (25%). To achieve laminar order in aggregates, at all ages, the addition of pigment epithelial cells seems to be a prerequisite.
我们在此报告，在鸡视网膜细胞的旋转培养中，尤其是视网膜和色素上皮细胞的混合物中，组织重建能力存在显著的年龄依赖性。乙酰胆碱酯酶组织化学染色后的切片比较得出了以下结果。在视网膜细胞聚集体中，可以清楚地观察到细胞分选现象，但仅能检测到轻微的年龄依赖性效应，例如，与其他阶段的聚集体相比，来自6日龄细胞的聚集体的层状排列有所增加。在视网膜和色素上皮细胞混合物的聚集体中，5日龄胚胎细胞形成的聚集体的层状排列程度最高，而在较大年龄时则显著降低。通过胚胎第5天的视网膜细胞重新聚集，获得了重建所有主要视网膜层的聚集体的最高数量（25%）。为了在聚集体中实现层状排列，在所有年龄段，添加色素上皮细胞似乎都是一个先决条件。
