Layer P G, Willbold E
Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Federal Republic of Germany.
Cell Tissue Res. 1989 Nov;258(2):233-42. doi: 10.1007/BF00239443.
The capacities of retinal and pigmented cells to regenerate histotypic in-vitro-retinae (IVR) in rotary culture were investigated by dividing the eye cups of 6-day-old chicken embryos into a central and a peripheral part; they were cut along the ora serrata, and the retinal and the pigmented constituents of both parts were isolated. The 4 dissociated cell populations were cultured separately and in all double combinations. Two different types of IVR's were generated; one developed from central or peripheral retinal cells, the other required the addition of pigmented cells from the ciliary margin of the eye. The shape of these IVR's was examined using scanning electron microscopy, and they were also characterized histologically. The acetylcholinesterase pattern marked the inner half of the retina; F11-antibody and a peanut agglutinin marker revealed both plexiform layers and a radial fiber system. In both types, organized histotypical areas consisted of complete sets of retinal layers. In the type containing pigmented cells from the eye periphery, the sequence of layers was identical with that of an in-situ-retina ("laminar IVR"). In IVR's derived from retinal cells only, the sequence of layers was reversed ("rosetted IVR").
通过将6日龄鸡胚的眼杯分为中央部分和周边部分，研究视网膜和色素细胞在旋转培养中再生组织型体外视网膜（IVR）的能力；沿着锯齿缘将它们切开，并分离两部分的视网膜和色素成分。将4个解离的细胞群体分别培养，并进行所有的双重组合培养。产生了两种不同类型的IVR；一种由中央或周边视网膜细胞发育而来，另一种需要添加来自眼睫状缘的色素细胞。使用扫描电子显微镜检查这些IVR的形状，并对它们进行组织学表征。乙酰胆碱酯酶模式标记了视网膜的内半部；F11抗体和花生凝集素标记物显示了两个神经纤维层和一个放射状纤维系统。在两种类型中，有组织的组织型区域均由完整的视网膜层组成。在含有来自眼周边色素细胞的类型中，层的顺序与原位视网膜相同（“层状IVR”）。在仅由视网膜细胞衍生的IVR中，层的顺序是相反的（“玫瑰花结状IVR”）。
