Fujita Y, Fujita T
Nucleic Acids Res. 1986 Feb 11;14(3):1237-52. doi: 10.1093/nar/14.3.1237.
The nucleotide sequence (742 bp) of the promoter region of the Bacillus subtilis gluconate (gnt) operon is presented. Nuclease Sl mapping revealed the start point of the transcription and suggested that the expression of this operon is probably regulated at the transcriptional level. The sequences of the -35 and -10 regions suggested that RNA polymerase possessing sigma-43 may recognize this structure. The 223 bp fragment containing 100 bp upstream from the transcription start site actually exhibited a promoter activity when cloned in a promoter probe vector of pPL603B. This promoter activity was highly derepressed and although still under catabolite repression. The fragment on a high copy plasmid could titrate a regulator of the gnt operon so that the expression of the operon on the host chromosome also became derepressed.
本文给出了枯草芽孢杆菌葡萄糖酸盐（gnt）操纵子启动子区域的核苷酸序列（742 bp）。核酸酶S1图谱分析揭示了转录起始点，并表明该操纵子的表达可能在转录水平受到调控。-35区和-10区的序列表明，含有σ-43的RNA聚合酶可能识别该结构。当克隆到pPL603B启动子探针载体中时，包含转录起始位点上游100 bp的223 bp片段实际上表现出启动子活性。该启动子活性高度去阻遏，尽管仍处于分解代谢物阻遏之下。高拷贝质粒上的片段可以滴定gnt操纵子的一个调节因子，从而使宿主染色体上操纵子的表达也去阻遏。
