Department of Medical Laboratory Science & Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
Future Microbiol. 2013 Nov;8(11):1381-9. doi: 10.2217/fmb.13.115.
Development of the matrix-assisted laser desorption ionization-TOF (MALDI-TOF) mass spectrometry method to rapidly identify Mycobacteria abscessus and Mycobacteria massiliense from M. abscessus complex in clinical microbiology laboratories.
MATERIALS & METHODS: Of 128 M. abscessus complex clinical isolates, sequence analysis identified 64 as M. massiliense and 64 as M. abscessus. MALDI-TOF mass spectrometry with clustering analysis created a model to differentiate these two species. Multilocus sequence typing was used to confirm our model.
Using a model containing five signals, 100% species recognition was achieved for 50 strains of each species. The sequence type (ST) cluster of M. abscessus was distinct from the cluster of M. massiliense. ST1 was prevalent (59%) among M. abscessus isolates, and ST117 was common (41%) among M. massiliense isolates.
Molecular methods are more costly and time-consuming and may not be available in the clinical microbial laboratory. Subsequently, MALDI-TOF analysis could be a rapid identification method in the future.
建立基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱法，用于快速鉴定临床微生物实验室中从脓肿分枝杆菌复合体中分离出的脓肿分枝杆菌和马赛分枝杆菌。
对 128 株脓肿分枝杆菌复合体临床分离株进行序列分析，鉴定出 64 株为马赛分枝杆菌，64 株为脓肿分枝杆菌。采用 MALDI-TOF 质谱和聚类分析创建了一种区分这两种物种的模型。多位点序列分型用于验证我们的模型。
使用包含五个信号的模型，对每个物种的 50 株菌株实现了 100%的物种识别。脓肿分枝杆菌的序列型（ST）聚类与马赛分枝杆菌的聚类明显不同。ST1 在脓肿分枝杆菌分离株中较为常见（59%），ST117 在马赛分枝杆菌分离株中较为常见（41%）。
分子方法成本更高，耗时更长，且可能无法在临床微生物实验室中使用。因此，MALDI-TOF 分析可能是未来的一种快速鉴定方法。
