Amrita center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham (University), Kochi, India.
Amrita center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham (University), Kochi, India.
Nanomedicine. 2014 Apr;10(3):579-87. doi: 10.1016/j.nano.2013.10.006. Epub 2013 Nov 4.
A multifunctional core-shell nanomedicine capable of inhibiting the migratory capacity of metastatic cancer cells followed by imparting cytotoxic stress by photodynamic action is reported. Based on in silico design, we have developed a core-shell nanomedicine comprising of ~80nm size poly(lactic-co-glycolic acid) (PLGA) nano-core encapsulating photosensitizer, m-tetra(hydroxyphenyl)chlorin (mTHPC), and ~20nm size albumin nano-shell encapsulating tyrosine kinase inhibitor, Dasatinib, which impair cancer migration. This system was prepared by a sequential process involving electrospray of polymer core and coacervation of protein shell. Cell studies using metastatic breast cancer cells demonstrated disruption of Src kinase involved in the cancer migration by albumin-dasatinib nano-shell and generation of photoactivated oxidative stress by mTHPC-PLGA nano-core. This unique combinatorial photo-chemo nanotherapy resulted synergistic cytotoxicity in ~99% of the motility-impaired metastatic cells. This approach of blocking cancer migration followed by photodynamic killing using rationally designed nanomedicine is a promising new strategy against cancer metastasis.
A multifunctional core-shell nanomedicine capable of inhibiting metastatic cancer cell migration, in addition to inducing photodynamic effects, is described in this paper. The authors document cytotoxicity in approximately 99% of the studied metastatic breast cancer cells. Similar approaches would be a very welcome addition to the treatment protocols of advanced metastatic breast cancer and other types of neoplasms.
本文报道了一种多功能核壳纳米医学，它能够抑制转移性癌细胞的迁移能力，然后通过光动力作用施加细胞毒性应激。基于计算机设计，我们开发了一种核壳纳米医学，它由80nm 大小的聚（乳酸-共-乙醇酸）（PLGA）纳米核包裹光敏剂 m-四（羟基苯基）氯（mTHPC）和20nm 大小的白蛋白纳米壳包裹酪氨酸激酶抑制剂达沙替尼组成，从而损害癌症迁移。该系统通过涉及聚合物核的电喷雾和蛋白质壳的凝聚的顺序过程制备。使用转移性乳腺癌细胞的细胞研究表明，白蛋白-达沙替尼纳米壳破坏了参与癌症迁移的Src 激酶，并通过 mTHPC-PLGA 纳米核产生光激活的氧化应激。这种独特的组合光化疗纳米治疗导致~99%的运动受损转移性细胞产生协同细胞毒性。这种阻断癌症迁移然后使用合理设计的纳米医学进行光动力杀伤的方法是对抗癌症转移的一种很有前途的新策略。
本文描述了一种多功能核壳纳米医学，它能够抑制转移性癌细胞的迁移，同时还能诱导光动力效应。作者记录了大约 99%的研究转移性乳腺癌细胞的细胞毒性。类似的方法将非常欢迎添加到晚期转移性乳腺癌和其他类型肿瘤的治疗方案中。
