Pavlenko I V, Krasheniuk A I, Sal'nikova T A, Brezgun A A, Smorodinova I P
Vopr Virusol. 1985 Nov-Dec;30(6):732-6.
A number of two-phase aqueous polymers based on dextran (mol. w. 500) and polyethylene glycol (mol. w. 1000, 4000, 6000) was studied. The two-step distribution of virus-containing tissue culture fluid in these systems was found to result in significant purification of measles virus from protein admixtures. The degree of virus purification was shown to depend on ratios of volumes and molecular weights of the polymers.
研究了多种基于葡聚糖(分子量500)和聚乙二醇(分子量1000、4000、6000)的双水相聚合物。发现在这些体系中含病毒的组织培养液的两步分配可显著从蛋白质混合物中纯化麻疹病毒。结果表明病毒纯化程度取决于聚合物的体积比和分子量。