Ibrahim Dalia, Nakamuta Nobuaki, Taniguchi Kazumi, Yamamoto Yoshio, Taniguchi Kazuyuki
United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
J Vet Med Sci. 2014 Mar;76(3):339-46. doi: 10.1292/jvms.13-0436. Epub 2013 Nov 8.
The olfactory and respiratory mucosae of the Corriedale sheep were examined using lectin histochemistry in order to clarify the histochemical and glycohistochemical differences between these two tissues. The olfactory epithelium was stained with 13 lectins out of 21 lectins examined, while the respiratory epithelium was positive to 16 lectins. The free border of both of the olfactory and respiratory epithelia was stained with 12 lectins: Wheat germ agglutinin (WGA), succinylated-wheat germ agglutinin (s-WGA), Lycopersicon esculentum lectin (LEL), Solanum tuberosum lectin (STL), Datura stramonium lectin (DSL), Soybean agglutinin (SBA), Bandeiraea simplicifolia lectin-I (BSL-I), Ricinus communis agglutinin-I (RCA-120), Erythrina cristagalli lectin (ECL), Concanavalin A (Con A), Phaseolus vulgaris agglutinin-E (PHA-E) and Phaseolus vulgaris agglutinin-L (PHA-L). The associated glands of the olfactory mucosa, Bowman's glands, were stained with 13 lectins. While both the goblet cells and mucous nasal glands were stained with 8 lectins; five of them (WGA, s-WGA, STL, Vicia villosa agglutinin (VVA) and ECL) were mutually positive among the Bowman's glands, mucous nasal glands and the goblet cells. These findings indicate that the glycohistochemical characteristics of the free borders of both olfactory and respiratory epithelia are similar to each other, suggesting that secretions from the Bowman's glands and those of the goblet cells and mucous nasal glands are partially exchanged between the surface of two epithelia to contribute the functions of the respiratory epithelium and the olfactory receptor cells, respectively.
为了阐明科里代尔羊的嗅觉黏膜和呼吸黏膜在组织化学和糖组织化学上的差异，采用凝集素组织化学方法对其进行了检查。在所检测的21种凝集素中，有13种凝集素可使嗅觉上皮染色，而呼吸上皮对16种凝集素呈阳性反应。嗅觉上皮和呼吸上皮的游离缘均被12种凝集素染色，分别是：麦胚凝集素（WGA）、琥珀酰化麦胚凝集素（s-WGA）、番茄凝集素（LEL）、马铃薯凝集素（STL）、曼陀罗凝集素（DSL）、大豆凝集素（SBA）、单叶豆凝集素-I（BSL-I）、蓖麻凝集素-I（RCA-120）、刺桐凝集素（ECL）、刀豆球蛋白A（Con A）、菜豆凝集素-E（PHA-E）和菜豆凝集素-L（PHA-L）。嗅觉黏膜的相关腺体——鲍曼腺，被13种凝集素染色。杯状细胞和鼻黏液腺均被8种凝集素染色；其中5种（WGA、s-WGA、STL、绒毛豌豆凝集素（VVA）和ECL）在鲍曼腺、鼻黏液腺和杯状细胞中呈共同阳性。这些结果表明，嗅觉上皮和呼吸上皮游离缘的糖组织化学特征彼此相似，这表明鲍曼腺的分泌物与杯状细胞和鼻黏液腺的分泌物在两种上皮表面之间部分交换，分别有助于呼吸上皮和嗅觉受体细胞的功能。
