Kim Seong-Min, Kim Jong-Min, Shin Dong-Gu, Kim Jae-Ryong, Cho Kyung-Hyun
School of Biotechnology, Aging-associated Vascular Disease Research Center, Yeungnam University, Gyeongsan 712-749, South Korea; Research Institute of Protein Sensor, Yeungnam University, Gyeongsan 712-749, South Korea.
Cardiovascular division, Internal Medicine, Aging-associated Vascular Disease Research Center, Yeungnam University Medical Center, Daegu 705-717, South Korea; Research Institute of Protein Sensor, Yeungnam University, Gyeongsan 712-749, South Korea.
Clin Biochem. 2014 Jul;47(10-11):869-75. doi: 10.1016/j.clinbiochem.2013.10.026. Epub 2013 Nov 4.
This study was designed to search putative biomarkers for detection of relatively young-onset atrial fibrillation (AF).
We analyzed serum lipoproteins from male patients with paroxysmal AF (48±9years old, n=29) and controls with similar age (50±10years old, n=27), who visited our hospital for radiofrequency catheter ablation due to paroxysmal supraventricular tachycardia.
Although the AF group showed normal serum cholesterol level, they exhibited 16% lower HDL-cholesterol and 13% higher serum cholesteryl ester transfer protein activity than those of the control group. The AF group showed elevated levels of serum triglyceride (TG) and C-reactive protein with hyperuricemia. However, there was no difference between serum levels of creatinine, troponin I, and serum amyloid A. All lipoproteins from the AF group contained higher level of TG, oxidized species, and advanced glycated end products. LDL from the AF group (AF-LDL) showed 2.7-fold more content of malondialdehyde than the control group (p<0.04) and exhibited higher sensitivity of oxidation. HDL-associated paraoxonase from the AF group showed impaired antioxidant ability and lowered expressional level of apoA-I (p<0.01) and paraoxonase (p<0.005) in HDL3.
Lipoprotein properties were severely impaired in the AF group with increased extent of oxidation and inflammation. The modified lipoprotein properties with impaired antioxidant functions can be used as a putative biomarker for prognostic detection for the relatively young onset AF.
本研究旨在寻找用于检测相对年轻发病的心房颤动（AF）的潜在生物标志物。
我们分析了因阵发性室上性心动过速前来我院进行射频导管消融的男性阵发性AF患者（48±9岁，n = 29）和年龄相仿的对照组（50±10岁，n = 27）的血清脂蛋白。
尽管AF组血清胆固醇水平正常，但与对照组相比，其高密度脂蛋白胆固醇水平低16%，血清胆固醇酯转运蛋白活性高13%。AF组血清甘油三酯（TG）和C反应蛋白水平升高，伴有高尿酸血症。然而，血清肌酐、肌钙蛋白I和血清淀粉样蛋白A水平在两组间无差异。AF组所有脂蛋白的TG、氧化产物和晚期糖基化终产物水平均较高。AF组的低密度脂蛋白（AF-LDL）丙二醛含量比对照组高2.7倍（p<0.04），且氧化敏感性更高。AF组高密度脂蛋白相关的对氧磷酶抗氧化能力受损，高密度脂蛋白3中载脂蛋白A-I（p<0.01）和对氧磷酶（p<0.005）的表达水平降低。
AF组脂蛋白性质严重受损，氧化和炎症程度增加。抗氧化功能受损的修饰脂蛋白性质可作为相对年轻发病AF预后检测的潜在生物标志物。
