Department of Botany, University of Georgia, 30602, Athens, GA, USA.
Theor Appl Genet. 1992 Aug;84(5-6):608-16. doi: 10.1007/BF00224159.
Fifty-two introgression lines (BC2F8) from crosses between two Oryza sativa parents and five accessions of O. officinalis were analyzed for the introgression of O. officinalis chromosome segments. DNA from the parents and introgression lines was analyzed with 177 RFLP markers located at approximately 10-cM intervals over the rice chromosomes. Most probe/enzyme combinations detected RFLPs between the parents. Of the 174 informative markers, 28 identified putative O. officinalis introgressed chromosome segments in 1 or more of the introgression lines. Introgressed segments were found on 11 of the 12 rice chromosomes. In most cases of introgression, O. sativa RFLP alleles were replaced by O. officinalis alleles. Introgressed segments were very small in size and similar in plants derived from early and later generations. Some nonconventional recombination mechanism may be involved in the transfer of such small chromosomal segments from O. officinalis chromosomes to those of O. sativa. Some of the introgressed segments show association with genes for brown planthopper (BPH) resistance in some introgressed lines, but not in others. Thus, none of the RFLP markers could be unambiguously associated with BPH resistance.
52 个来自两个水稻亲本与五个长芒野生稻导入系杂交的导入系（BC2F8）被分析其导入的长芒野生稻染色体片段。来自亲本和导入系的 DNA 用 177 个 RFLP 标记进行分析，这些标记大约以 10-cM 的间隔分布在水稻染色体上。大多数探针/酶组合在亲本之间检测到 RFLPs。在 174 个有信息的标记中，28 个标记在 1 个或更多的导入系中鉴定出可能的长芒野生稻导入染色体片段。在 12 条水稻染色体中的 11 条上发现了导入片段。在大多数导入情况下，水稻的 RFLP 等位基因被长芒野生稻等位基因取代。导入片段的大小非常小，在早期和后期世代衍生的植物中相似。可能涉及一种非传统的重组机制，将如此小的染色体片段从长芒野生稻染色体转移到水稻染色体上。一些导入片段与一些导入系中褐飞虱（BPH）抗性基因有关，但在其他导入系中没有。因此，没有一个 RFLP 标记可以与 BPH 抗性明确相关。
