Department of Agronomy, University of Illinois, Turner Hall, 1102 S Goodwin Ave., 61801, Urbana, IL, USA.
Theor Appl Genet. 1992 Aug;84(5-6):688-92. doi: 10.1007/BF00224169.
Total seed storage protein of the cultivated chickpea, C. arietinum L., and eight other wild annual Cicer species (all 2n = 16) was separated and compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The seed-protein profile was a conservative and species-specific trait. Relative interspecific similarities of protein patterns were estimated using Jaccard's similarity index, and a cluster analysis was performed. The resultant dendrogram generally agreed with the limited data already available on interspecific relationships in Cicer based on morphological characteristics, crossability, genome pairing in hybrids, karyotypes and isozyme analysis. The difference between the profiles of C. judaicum and C. pinnatifidum supported the idea that they are indeed two separate species. The closest relative of C. arietinum was C. reticulatum, followed by C. echinospermum and other species, while C. cuneatum was the farthest relative. In general, C. cuneatum was also genetically the farthest removed from any other species. The suggestion that C. reticulatum is the wild progenitor of the cultivated chickpea was therefore further supported.
栽培鹰嘴豆（C. arietinum L.）和其他八种野生一年生鹰嘴豆属（均为 2n=16）的总种子贮藏蛋白通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳进行分离和比较。种子蛋白图谱是一种保守的、种特异性的特征。使用雅可比相似性指数估计蛋白质图谱的相对种间相似性，并进行聚类分析。基于形态特征、可杂交性、杂种基因组配对、染色体组型和同工酶分析，所得的聚类图与已有的关于鹰嘴豆属种间关系的有限数据基本一致。C. judaicum 和 C. pinnatifidum 图谱的差异支持它们确实是两个独立物种的观点。C. arietinum 的近亲是 C. reticulatum，其次是 C. echinospermum 和其他物种，而 C. cuneatum 是最远的亲缘关系。总的来说，C. cuneatum 在遗传上也与其他任何物种的关系最远。因此，进一步支持 C. reticulatum 是栽培鹰嘴豆的野生祖先的观点。
