Division of Genetics, Indian Agricultural Research Institute, New Delhi, India.
Theor Appl Genet. 1972 Jan;42(8):351-6. doi: 10.1007/BF00275360.
Types representing three subspecies of Oryza sativa, namely, indica, japonica and javanica, and a group of intermediate types collected from North East India, were studied for variation in soluble proteins using acrylamide gel electrophoresis. The study revealed that there was a marked variation within and between varietal groups. Variability for number and intensity of protein bands in indica was wider than in japonica and javanica. Protein pattern in the group comprising N.E. Indian types transgressed that of all three established groups by displaying a wide spectrum. Relative homology, as measured from percentage similarities of N.E. Indian types to the three subspecies, suggested the existence of six different groups. Comparison of varietal groups for the protein mobility pattern showed that japonica and javanica varieties tended to show higher percentages of slow mobility proteins than indica. It appears, from the narrow variability and relatively low percentage of slow mobility proteins, that the japonica and javanica races are of later origin compared to indica. However, the study with a limited number of types suggested the monophyletic origin of varietal groups from an indica-like base predominantly found in the secondary centres of origin of O. sativa.
利用聚丙烯酰胺凝胶电泳技术，对代表三个亚种（籼稻、粳稻和爪哇稻）的类型以及从印度东北部收集的一组中间类型的水稻进行了可溶性蛋白变异的研究。研究表明，种内和种间均存在明显的变异。籼稻的蛋白带数量和强度的变异性大于粳稻和爪哇稻。由印度东北部类型组成的组的蛋白图谱通过显示广泛的谱超过了所有三个已建立的组。从印度东北部类型与三个亚种的相似百分比测量的相对同源性表明存在六个不同的组。对蛋白泳动模式的品种群比较表明，与籼稻相比，粳稻和爪哇稻品种往往表现出更高比例的慢移动蛋白。从狭窄的变异性和相对较低的慢移动蛋白百分比来看，似乎粳稻和爪哇稻种的起源比籼稻晚。然而，这项具有有限数量类型的研究表明，品种群的单系起源主要来自籼稻，籼稻在水稻的次要起源中心发现。
