Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA.
Theor Appl Genet. 1996 Feb;92(2):245-54. doi: 10.1007/BF00223381.
In order to determine the pattern of genetic diversity within and among the species of Cicer and to estimate interspecific genetic relationships, allelic variation was assayed for 23 isozyme loci in 63 accessions of 11 species of Cicer using starch gel electrophoresis. The total allozymic variation observed in the genus (H t )was equal to 0.60. When partitioned (G st), 96% of this allelic diversity was found among rather than within species. The allelic diversity among species (D st)and allelic diversity within species (H s)were equal to 0.58 and 0.02, respectively. Cicer reticulatum and C. pinnatifidum had the highest proportion of polymorphic loci (17.39%) and the highest mean number of alleles per locus (1.22 and 1.17, respectively). UPGMA cluster analysis of Nei's unbiased genetic distance revealed four genetic groups. One includes C. reticulatum, C. arietinum and C. echino spermum where the first 2 species represent a putative derivative-progenitor pair. A second cluster contains C. bijugum, C. pinnatifidum and C. judaicum. Cicer yamashitae, C. chorassanicum, C. anatolicum and C. songoricum form a third group. Finally, C. cuneatum, which has a very distinct isozyme profile and peculiar morphological features, is the only member of a fourth species group. This species grouping agrees partially with those obtained from crossability and cytogenetic studies. The results suggest that the annual habit arose from perennial progenitors at least twice in the genus Cicer.
为了确定鹰嘴豆属内和种间的遗传多样性模式，并估计种间的遗传关系，使用淀粉凝胶电泳检测了 11 种 63 个鹰嘴豆属材料的 23 个同工酶基因座的等位基因变异。在该属中观察到的总等位基因变异（Ht）等于 0.60。当划分时（Gst），96%的等位基因多样性存在于种间而不是种内。种间等位基因多样性（Dst）和种内等位基因多样性（Hs）分别等于 0.58 和 0.02。皱粒鹰嘴豆和宽翅鹰嘴豆具有最高比例的多态性基因座（17.39%）和每个基因座的平均等位基因数（1.22 和 1.17，分别）。基于 Nei 无偏遗传距离的 UPGMA 聚类分析揭示了 4 个遗传群体。第一个群体包括皱粒鹰嘴豆、普通鹰嘴豆和刺果鹰咀豆，前 2 个种代表一个假定的衍生祖先对。第二个群体包含二棱鹰嘴豆、宽翅鹰嘴豆和野豌豆鹰嘴豆。Cicer yamashitae、C.chorassanicum、C. anatolicum 和 C. songoricum 形成第三个群体。最后，C. cuneatum 是第四个种群体的唯一成员，它具有非常独特的同工酶图谱和特殊的形态特征。这种物种分组与通过可交配性和细胞遗传学研究获得的分组部分一致。结果表明，在鹰嘴豆属中，一年生习性至少从多年生祖先中两次进化而来。
