Pelletier G, Labrie F, Kastin A J, Coy D, Schally A V
Pharmacol Biochem Behav. 1975 Jul-Aug;3(4):675-9. doi: 10.1016/0091-3057(75)90191-4.
Tritiated L-prolyl-L-leucyl-glycinamide was injected into the carotid artery or lateral ventricle of rats and the radioactivity localized by radioautography. After intracarotid administration, the radioactivity labeled the meninges, ependymal cells bordering the ventricles, and cells of the choroid plexus, as well as the median eminence and subfornical organ and thus may have reached the cerebrospinal fluid. After intraventricular injection, globus pallidus, indusium griseum, hippocampus, corpus callosum, and meninges. The results after intracarotid injection suggest that L-prolyl-L-leucyl-glycinamide may cross the blood brain barrier and the results after intraventricular injection suggest sites of localization which might be correlated with the previously reported effects of the tripeptide on the brain.
将氚标记的L-脯氨酰-L-亮氨酰-甘氨酰胺注入大鼠的颈动脉或侧脑室，并通过放射自显影定位放射性。颈动脉内给药后，放射性标记了脑膜、脑室周围的室管膜细胞、脉络丛细胞，以及正中隆起和穹窿下器官，因此可能进入了脑脊液。脑室内注射后，放射性标记了苍白球、灰被、海马、胼胝体和脑膜。颈动脉内注射后的结果表明L-脯氨酰-L-亮氨酰-甘氨酰胺可能穿过血脑屏障，脑室内注射后的结果表明了放射性定位的部位，这些部位可能与先前报道的该三肽对大脑的作用相关。
