Rees H D, Verhoef J, Witter A, Gispen W H, de Wied D
Brain Res Bull. 1980 Sep-Oct;5(5):509-14. doi: 10.1016/0361-9230(80)90255-5.
Autoradiographic studies aimed at identifying target cells in the brain for ACTH-like peptides were performed using (3H-7-Phe)-4-Met(O2),8-D-Lys, 9-Phe-ACTH4--9, a behaviorally potent analog of ACTH4--9. The 3H-peptide was injected into the lateral ventricle of hypophysectomized rats that were sacrificed 5, 30, 60, 180, and 240 min later. Dry-mount autoradiograms of brain showed the highest density of silver grains in the ventricular lumen and choroid plexus. In addition, radioactivity penetrated brain tissue as far as 100 microns from the ventricles, and was distributed predominantly over neuropil. Within 5 min after the injection, an intracellular concentration of radioactivity above background levels was observed in a small proportion of cells near the ventricles in the septum, caudate-putamen, preoptic area, hypothalamus, thalamus, amygdala, and hippocampus. The cellular labeling decreased in intensity at greater distances from the injection site and at longer survival intervals, and was no longer evident 4 hr after the injection. The labeled cells were usually small, dark, and often elongated, suggesting that ACTH peptides may act preferentially upon a morphologically distinct class of cells in the brain.
使用促肾上腺皮质激素4-9的行为活性类似物（3H-7-苯丙氨酸）-4-甲硫氨酸（O2），8-D-赖氨酸，9-苯丙氨酸-促肾上腺皮质激素4-9进行放射自显影研究，以确定大脑中促肾上腺皮质激素样肽的靶细胞。将3H肽注入垂体切除大鼠的侧脑室，5、30、60、180和240分钟后处死大鼠。大脑的干装放射自显影片显示脑室腔和脉络丛中银颗粒密度最高。此外，放射性物质穿透脑组织至距脑室100微米处，主要分布在神经毡上。注射后5分钟内，在隔膜、尾状核-壳核、视前区、下丘脑、丘脑、杏仁核和海马体脑室附近的一小部分细胞中观察到细胞内放射性浓度高于背景水平。在距注射部位更远的距离和更长的存活时间间隔下，细胞标记强度降低，注射后4小时不再明显。标记的细胞通常较小、颜色深且常呈细长形，这表明促肾上腺皮质激素肽可能优先作用于大脑中一类形态上不同的细胞。
