Department of Pharmacognosy, University of Geneva, Sciences II, 30, Quai E.-Ansermet, CH-1211, 4, Geneva, Switzerland.
Plant Cell Rep. 1992 Jul;11(7):339-42. doi: 10.1007/BF00233361.
Hairy root cultures of Valeriana officinalis var. sambucifolia were established by infection of sterile plantlets with Agrobacterium rhizogenes strain R1601 The transformed roots were grown in 10 different, hormone-free liquid media and the isovaltrate, valtrate, didrovaltrate, isovaleroxyhydroxydidrovaltrate content was quantified by HPLC. Valepotriates were entirely retained inside the root tissues. The highest overall valepotriate content (10.3 % dry wt), 4 times the amount found in the roots of 9-month-old nontransformed plants, was observed in half strength Gamborg B5 medium supplemented with 2 % sucrose. The hairy roots cultured in Murashige and Skoog liquid medium supplemented with 2 % sucrose for 50 days produced over 44 mg/g dry wt valepotriates.
毛状根培养的缬草变种 sambucifolia 通过感染无菌植物与发根农杆菌菌株 R1601。转化的根生长在 10 种不同的、无激素的液体培养基中，并用高效液相色谱法定量异缬草酸、缬草酸、二氢缬草酸、异缬草酸羟氢缬草酸的含量。缬草酯完全保留在根组织内。在补充有 2%蔗糖的半强度 Gamborg B5 培养基中观察到最高的总缬草酯含量（10.3%干重），是 9 个月大的未转化植物根中含量的 4 倍。在补充有 2%蔗糖的 Murashige 和 Skoog 液体培养基中培养的毛状根 50 天产生超过 44mg/g 干重的缬草酯。
