Tavassoli Parisa, Safipour Afshar Akbar
Biology Department, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
3 Biotech. 2018 Aug;8(8):351. doi: 10.1007/s13205-018-1375-z. Epub 2018 Jul 31.
Hairy roots were induced in using , strains A4, A13, ATCC15834, and ATCC15834. The leaf, petiole and shoot explants of marshmallow were used for the hairy roots induction. When hairy roots appeared, cultures were established in MS (Murashige and Skoog) liquid medium without growth regulators. Hairy roots in explants appeared 5-12 days after inoculation. Maximum transformation frequency of 83% was observed on shoot explants with ATCC15834 strain. Among the strains, ATCC15834 strain showed better potential in the mass production of hairy roots in the hormone-free liquid medium after 50 days of culturing. The highest total phenolic and flavonoids content was found at 1.57 ± 0.1 mg/g dry weight in A13 strain and 3.47 ± 0.3 mg/g in A4 strain, respectively. Secondary metabolite content of hairy roots was found to be strain-specific.
使用发根农杆菌A4、A13、ATCC15834菌株诱导出了毛状根。以药蜀葵的叶片、叶柄和茎段外植体用于毛状根诱导。当毛状根出现后，将培养物接种于不含生长调节剂的MS（Murashige和Skoog）液体培养基中。接种后5 - 12天，外植体上出现毛状根。在茎段外植体上使用ATCC15834菌株时，观察到最高转化频率为83%。在这些菌株中，培养50天后，ATCC15834菌株在无激素液体培养基中大量生产毛状根方面表现出更好的潜力。A13菌株中总酚含量最高，为1.57±0.1毫克/克干重，A4菌株中黄酮类化合物含量最高，为3.47±0.3毫克/克。发现毛状根的次生代谢产物含量具有菌株特异性。
