玉米中的基质相关 DNA 在重复序列中富集。

Matrix-associated DNA from maize is enriched in repetitive sequences.

机构信息

Institute of Genetics, Bulgarian Academy of Sciences, PO Box 96, 1113, Sofia, Bulgaria.

出版信息

Plant Cell Rep. 1992 Jul;11(7):355-8. doi: 10.1007/BF00233365.

PMID:24201438

Abstract

In order to elucidate some features of the topological organization of DNA within the plant nucleus, DNA fragments involved in the attachment of the DNA loops to the nuclear matrix in maize were studied. The matrix-associated DNA from dry embryo and meristematic cells after extensive digestion with DNase I and high salt treatment was about 2% of the total DNA, sized within the range of 50 and 250 bp. This DNA was found to be enriched in repetitive DNA sequences, both for nuclei from dry embryo and meristematic cells. The loop size of the DNA in cells of Zea mays appeared to be between 5 and 25 kbp.

摘要

为了阐明植物细胞核内 DNA 拓扑组织的一些特征，研究了与 DNA 环附着到玉米核基质的 DNA 片段。用 DNase I 和高盐处理充分消化后，干胚胎和分生组织细胞的基质相关 DNA 约占总 DNA 的 2%，大小在 50 和 250 bp 之间。该 DNA 富含重复 DNA 序列，无论是来自干胚胎还是分生组织细胞的核。玉米细胞中的 DNA 环大小似乎在 5 到 25 kbp 之间。