Mirkovitch J, Mirault M E, Laemmli U K
Cell. 1984 Nov;39(1):223-32. doi: 10.1016/0092-8674(84)90208-3.
Data are presented for sequence-specific chromatin-loop organization in histone-depleted nuclei from Drosophila melanogaster Kc cells. We find one loop for each of the tandemly repeated histone gene clusters. The attachment site is localized in the A + T rich H1-H3 spacer on a 657 bp fragment. In the cluster of the hsp70 heat-shock genes, in both control and heat-shocked cells, we find two attachment sites in close proximity upstream of regulatory elements. The transcribed sequences are not associated with the nuclear scaffold in control or in heat-shocked cells. A family of attachment sites related by hybridization to those of the hsp70 genes was discovered.
本文展示了来自黑腹果蝇Kc细胞的组蛋白缺失细胞核中序列特异性染色质环的组织情况。我们发现每个串联重复的组蛋白基因簇都有一个环。附着位点位于一个657 bp片段上富含A + T的H1 - H3间隔区。在hsp70热休克基因簇中，无论是对照细胞还是热休克细胞，我们在调控元件上游紧邻位置发现了两个附着位点。在对照细胞或热休克细胞中，转录序列都不与核支架相关联。发现了一个通过杂交与hsp70基因的附着位点相关的附着位点家族。
