Department of Breeding, Hokuriku National Agricultural Experiment Station, 943-01, Joetsu, Japan.
Theor Appl Genet. 1992 Sep;84(7-8):787-91. doi: 10.1007/BF00227385.
Rice and barley chromosomal samples were prepared both on a polyester membrane and on an ordinary glass slide and subjected to microdissection by an argon-ion laser. The intensity and the position of the laser beam were controlled by a microcomputer. The most suitable intensity to obtain chromosomal fragments was determined experimentally. As a result, specific regions of the centromere, satellite, short arm, or long arm, of the barley and rice chromosomes were dissected out from the chromosomal spreads. Chromosomal fragments were also successfully transferred from the sample into an Eppendorf tube.
水稻和大麦染色体样本分别在聚酯膜和普通载玻片上制备，并用氩离子激光进行显微切割。激光束的强度和位置由微机控制。通过实验确定了获得染色体片段的最佳强度。结果，从染色体铺片中切取了大麦和水稻染色体着丝粒、卫星、短臂或长臂的特定区域。染色体片段也成功地从样本转移到了 Eppendorf 管中。
