Ponelies N, Bautz E K, Monajembashi S, Wolfrum J, Greulich K O
Physikalisch-chemisches Institut der Universität, Heidelberg, Federal Republic of Germany.
Chromosoma. 1989 Nov;98(5):351-7. doi: 10.1007/BF00292388.
Telomeric fragments from salivary gland squashes of Drosophila melanogaster Oregon R. were produced by a new microdissection technique, UV laser microbeam dissection. Microdissection, an essential step in microcloning procedures, is usually performed using micromanipulators and microneedles. Recently it has been shown that microdissection can be improved to very high precision if a laser coupled into a microscope is used. A laser microbeam, generated by an excimer pumped dye laser, allows chromosomes to be cut into slices of less than 0.5 micron. Here it is shown, that single copy DNA probes prepared from Drosophila chromosomes by laser microdissection and microcloning relocalize to the chromosomal regions from which they are derived. The combination of laser technique and microcloning provides an advantageous approach for rapid genetic analysis with potential for the study of genetic diseases and genome mapping.
利用一种新的显微切割技术——紫外激光微束切割，从黑腹果蝇俄勒冈R品系的唾液腺压片中获得了端粒片段。显微切割是微克隆程序中的关键步骤，通常使用显微操作器和微针来进行。最近研究表明，如果使用耦合到显微镜中的激光，显微切割的精度可以提高到非常高的水平。由准分子泵浦染料激光产生的激光微束能够将染色体切割成小于0.5微米的片段。本文展示了，通过激光微切割和微克隆从果蝇染色体制备的单拷贝DNA探针能够重新定位到它们所源自的染色体区域。激光技术与微克隆的结合为快速遗传分析提供了一种有利的方法，具有研究遗传疾病和基因组图谱的潜力。
