Brassica and Oilseed Research Department, Cambridge Laboratory, John Innes Centre, Colney Lane, NR4 7UJ, Norwich, UK.
Plant Cell Rep. 1992 Jul;11(8):408-11. doi: 10.1007/BF00234371.
Moricandia arvensis is of interest as it is a dicotyledonous species which has C3 - C4 intermediate photosynthesis, a mechanism which results in enhanced recapture of photorespired CO2. Leaves from cultured shoot tips were used as a source for mesophyll cell protoplasts. Approximately 1% of the protoplasts which survived the first few days of culture produced calli. On a suitable regeneration medium, 30-60% of the calli regenerated one or more shoots. From among the regenerating shoots eight were selected, transferred to soil and grown to flowering in the glasshouse; all were fertile. The development of a protoplast regeneration system provides the opportunity to use transformation and somaclonal variation as tools in the genetic analysis of the C3-C4 character in this species.
田蓟是一种感兴趣的物种，因为它是一种具有 C3-C4 中间光合作用的双子叶植物，这种机制导致了光呼吸 CO2 的增强回收。从培养的茎尖叶片中提取叶肉细胞原生质体。大约有 1%的原生质体在培养的最初几天存活下来，形成愈伤组织。在合适的再生培养基上，30-60%的愈伤组织再生出一个或多个芽。在再生芽中选择了 8 个，转移到土壤中，在温室中生长到开花；全部都是可育的。原生质体再生系统的发展为利用转化和体细胞变异作为该物种 C3-C4 特征遗传分析的工具提供了机会。
