Brassica and Oilseed Research Department, Cambridge Laboratory, John Innes Centre, Colney Lane, NR4 7UJ, Norwich, UK.
Plant Cell Rep. 1992 Jul;11(8):412-5. doi: 10.1007/BF00234372.
Techniques have been developed for the regeneration of Moricandia arvensis from complex explants. Hypocotyl segments and cotyledonary explants regenerated shoots, but the most efficient plant regeneration was from stem sections taken from in vitro grown shoots. Regeneration from these three explant types was tested on a range of concentrations of benzylaminopurine and either naphthylene acetic acid or indole acetic acid. Regeneration from all three explants was much higher on indole acetic acid than on naphthylene acetic acid and the ratio of auxin to cytokinin was also significant in determining the response of explants. Optimum regeneration was on 1mg/l IAA with 1mg/l BAP. Plants could be transferred to soil and grown to flowering in the glasshouse.
已经开发出了从复杂外植体再生野芝麻的技术。下胚轴切段和子叶外植体再生出了芽，但最有效的植株再生来自于离体生长的芽的茎段。这三种外植体类型在不同浓度的苄氨基嘌呤和萘乙酸或吲哚乙酸上进行了再生测试。与萘乙酸相比，三种外植体在吲哚乙酸上的再生率都要高得多，而生长素与细胞分裂素的比例在决定外植体的反应方面也很重要。最适再生条件为 1mg/L IAA 和 1mg/L BAP。植物可以转移到土壤中，并在温室中生长开花。
