Institute of Molecular Genetics, USSR Academy of Sciences, 123182, Moscow, USSR.
Plant Cell Rep. 1989 May;8(5):300-2. doi: 10.1007/BF00274135.
Plants were regenerated from cotyledon and hypocotyl explants of watermelon (Citrullus vulgaris). The explants were cultured on a Murashige and Skoog's basal nutrient medium supplemented with auxin, cytokinin and auxin-cytokinin combinations. Green healthy nodular and compact callus was obtained in medium containing naphthalene acetic acid and benzylaminopurine. Shoot differentiation and root differentiation from the cotyledon and hypocotyl after callus formation in different media containing benzylaminopurine or naphthalene acetic acid, respectively. Shoot formation required benzylaminopurine. Kinetin proved ineffective in inducing shoot buds or shoots. Root differentiation occurred in a medium containing naphthalene acetic acid or indole acetic acid. There was a greater proliferation of roots on medium supplemented with naphthalene acetic acid. The regenerated shoots developed roots when transferred to medium containing naphthalene acetic acid and complete plantlets could be transferred to soil for further growth.
从西瓜（Citrullus vulgaris）子叶和下胚轴外植体再生出植株。将外植体在添加了生长素、细胞分裂素和生长素-细胞分裂素组合的 Murashige 和 Skoog 基础营养培养基上培养。在含有萘乙酸和苄氨基嘌呤的培养基中获得了绿色健康的结节状和致密的愈伤组织。在含有苄氨基嘌呤或萘乙酸的不同培养基中，子叶和下胚轴形成愈伤组织后，分别进行芽和根的分化。芽的形成需要苄氨基嘌呤。激动素在诱导芽或芽方面无效。根分化发生在含有萘乙酸或吲哚乙酸的培养基中。在添加萘乙酸的培养基上，根的增殖更多。当再生的芽转移到含有萘乙酸的培养基中时，它们会生根，并且可以将完整的植株转移到土壤中进一步生长。
