Scottish Crop Research Institute, DD2 5DA, Invergowrie, Dundee, Scotland.
Plant Cell Rep. 1992 Aug;11(9):466-9. doi: 10.1007/BF00232692.
Randomly Amplified Polymorphic DNAs were employed to demonstrate that potato dihaploids generated after interspecific pollination of a tetraploid Solanum tuberosum cultivar (Pentland Crown) by Solanum phureja dihaploid inducer clones could not be of parthenogenetic origin. Of six different 10-mer oligonucleotides, four generated products from total potato dihaploid genomic DNAs which were not derived from the S. tuberosum parent. Gel electrophoresis and Southern analysis indicated that these amplified bands originated from S. phureja. The results are discussed in the context of recent cytological and molecular evidence which demonstrates that potato dihaploids are aneusomatic (Clulow et al. 1991) and emphasises this approach as a general methodology for the detection of alien gene introgression in both natural and cultivated plant populations.
随机扩增多态性 DNA 被用来证明，在四倍体马铃薯品种（Pentland Crown）与二倍体马铃薯诱导系杂种授粉后产生的马铃薯双单倍体，不可能是单性生殖的产物。在 6 种不同的 10 聚核苷酸中，有 4 种从总马铃薯双单倍体基因组 DNA 中产生了产物，而这些产物不是来自马铃薯双亲本。凝胶电泳和 Southern 分析表明，这些扩增带源自 S. phureja。该结果在最近的细胞学和分子证据的背景下进行了讨论，这些证据表明马铃薯双单倍体是异源体（Clulow 等人，1991 年），并强调了这种方法作为一种通用的方法，用于检测自然和栽培植物群体中外源基因的渗入。
