Biology Department, Washington University, 63130, St. Louis, MO, USA.
Planta. 1989 Oct;179(3):381-6. doi: 10.1007/BF00391084.
The unicellular alga Chlamydomonas reinhardtii Dang, has a cell wall made entirely from hydroxyproline-rich glycoproteins (HRGPs). We recently employed a quantiative in vitro reconstitution system (Adair et al. 1987, J. Cell Biol. 105, 2373-2382) to assign outer-wall HRGPs of C. reinhardtii to specific sublayers, and describe the major interactions responsible for their assembly. Some of these interactions appear to involve relatively conserved HRGP domains, as evidenced by interspecific cell-wall reconstitution between C. reinhardtii and two multicellular Volvocales (Volvoxcarteri lyengar and Gonium pectorale Müller). In the present report we provide biochemical and immunological evidence that the outer cell-walls of V. carteri and G. pectorale both contain prominent HRGPs closely related to C. reinhardtii GP2. Identification of conserved GP2 homologues indicates a molecular basis for interspecific reconstitution and provides a useful avenue for characterization of HRGP domains mediating cell-wall formation in these algae.
单细胞藻类莱茵衣藻（Chlamydomonas reinhardtii）的细胞壁完全由富含羟脯氨酸的糖蛋白（HRGPs）组成。我们最近采用了一种定量的体外重组系统（Adair 等人，1987 年，J. Cell Biol. 105，2373-2382），将莱茵衣藻的外壁 HRGPs 分配到特定的亚层，并描述了负责其组装的主要相互作用。这些相互作用中的一些似乎涉及相对保守的 HRGP 结构域，这一点可以从莱茵衣藻与两种多细胞绿藻（衣藻（Volvox carteri）和尾丝藻（Gonium pectorale））之间的种间细胞壁重组得到证明。在本报告中，我们提供了生化和免疫学证据，表明衣藻和尾丝藻的外壁都含有与莱茵衣藻 GP2 密切相关的突出的 HRGPs。保守的 GP2 同源物的鉴定表明了种间重组的分子基础，并为鉴定介导这些藻类细胞壁形成的 HRGP 结构域提供了有用的途径。
