Miller D H, Mellman I S, Lamport D T, Miller M
J Cell Biol. 1974 Nov;63(2 Pt 1):420-9. doi: 10.1083/jcb.63.2.420.
Cell walls of Chlamydomonas gymnogama, shed during sexual mating, were collected and analyzed. Ultrastructural examination indicates that the walls are free of cytoplasmic contamination and that they exhibit a regular lamellate structure. The walls are composed of glycoprotein rich in hydroxyproline. The hydroxyproline is linked glycosidically to a mixture of heterooligosaccharides composed of arabinose and galactose. Altogether, the glycoprotein complex accounts for at least 32% of the wall. The amino acid composition of the walls is extraordinarily similar in widely different plant species. The implications of these similarities as well as the widespread occurrence of these glycoproteins are discussed.
收集并分析了在有性交配过程中脱落的裸藻细胞壁。超微结构检查表明,这些细胞壁没有细胞质污染,并且呈现出规则的层状结构。细胞壁由富含羟脯氨酸的糖蛋白组成。羟脯氨酸通过糖苷键与由阿拉伯糖和半乳糖组成的杂寡糖混合物相连。总的来说,糖蛋白复合物至少占细胞壁的32%。在广泛不同的植物物种中,细胞壁的氨基酸组成异常相似。讨论了这些相似性的意义以及这些糖蛋白的广泛存在。