Max-Planck-Institut für Züchtungsforschung, Carl von Linne Weg 10, D-50837, Köln, Germany.
Plant Cell Rep. 1993 Aug;12(10):551-4. doi: 10.1007/BF00233058.
Growth characteristics of tobacco protoplasts containing rolA linked to its own promoter, or the rolB, or rolC genes of Agrobacterium rhizogenes linked to the Cauliflower Mosaic Virus 35S RNA promoter were compared with those from untransformed plants. RolA protoplasts require auxin and cytokinin for callus formation. Protoplasts overexpressing rolB and C form callus in the absence of exogenously applied auxin and cytokinin, respectively. Long term callus growth requires auxin, but the requirement for cytokinin is not critical. Optimal transient expression of an auxin responsive promoter element occurred at lower external levels of auxin in rolB and rolC protoplasts compared with untransformed protoplasts. Addition of putrescine was required for auxin responsive transient gene expression in rolA protoplasts suggesting that polyamines, or their products affect gene expression in rolA plants.
将 rolA 基因（与自身启动子相连）、rolB 基因或 rolC 基因（与花椰菜花叶病毒 35S RNA 启动子相连）连接到发根农杆菌中的烟草原生质体的生长特性与未转化植物的生长特性进行了比较。rolA 原生质体的愈伤组织形成需要生长素和细胞分裂素。分别过量表达 rolB 和 rolC 的原生质体在不添加外源生长素和细胞分裂素的情况下形成愈伤组织。长期的愈伤组织生长需要生长素，但细胞分裂素的需求并不关键。与未转化的原生质体相比，rolB 和 rolC 原生质体中生长素应答启动子元件的瞬时表达在较低的外部生长素水平下达到最佳。在 rolA 原生质体中添加腐胺对于生长素应答的瞬时基因表达是必需的，这表明多胺或其产物会影响 rolA 植物中的基因表达。
