John M, Schmidt J, Walden R, Czaja I, Dülz M, Schell J, Röhrig H
Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Cologne, Germany.
Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10178-82. doi: 10.1073/pnas.94.19.10178.
Lipochitooligosaccharides (LCOs) are plant growth regulators that promote at subfemtomolar concentrations cell division in tobacco protoplasts. In response to LCO treatment, tobacco cells release a second growth factor that fully mediates the growth-promoting activities of the initial extracellular LCO stimulus. This diffusible growth factor was isolated from the protoplasts' culture filtrate and shown to be a peptide. We report that the LCO-induced mitogen released by tobacco cells and a synthetic heptadecapeptide derived from region 2 of the tobacco homolog of the early nodulin gene ENOD40 are antigenically related and qualitatively indistinguishable in their ability to stimulate cell division.
脂壳寡糖（LCOs）是一种植物生长调节剂，在亚飞摩尔浓度下就能促进烟草原生质体的细胞分裂。响应LCO处理时，烟草细胞会释放出第二种生长因子，该因子能完全介导最初细胞外LCO刺激的促生长活性。这种可扩散的生长因子是从原生质体的培养滤液中分离出来的，并且已证明是一种肽。我们报告称，烟草细胞释放的LCO诱导有丝分裂原与源自早期结瘤素基因ENOD40烟草同源物区域2的合成十七肽在抗原性上相关，并且在刺激细胞分裂的能力上在质量上无法区分。
