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花生幼胚再生植株。

Plant regeneration from immature embryos of peanut.

机构信息

Department of Horticulture, University of Georgia, Coastal Plain Experiment Station, 31793, Tifton, GA, USA.

出版信息

Plant Cell Rep. 1989 Apr;8(4):217-8. doi: 10.1007/BF00778536.

DOI:10.1007/BF00778536
PMID:24233140
Abstract

Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal medium. Shoots were excised from thick embryonic roots and rerooted on Murashige and Skoog medium containing half the normal concentration of inorganic salts. This technique should be useful for the production of interspecific hybrid plants from immatureArachis embryos.

摘要

花生(Arachis hypogaea L.)未成熟胚再生可以通过体细胞胚胎发生来实现。体细胞胚形成的最高频率发生在 B5 培养基中加入 0.5-1.0 mg/l picloram。只有在基本培养基上延长培养后,才能从体细胞胚中发育出芽和植株。从厚的胚根中切下芽,然后在含有正常无机盐浓度一半的 Murashige 和 Skoog 培养基上重新生根。该技术应该对从未成熟的花生胚胎生产种间杂种植物有用。

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本文引用的文献

1
Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.).豌豆体细胞胚胎发生再生。
Plant Cell Rep. 1987 Jul;6(4):305-8. doi: 10.1007/BF00272005.
2
Nutrient requirements of suspension cultures of soybean root cells.大豆根细胞悬浮培养的营养需求。
Exp Cell Res. 1968 Apr;50(1):151-8. doi: 10.1016/0014-4827(68)90403-5.
花生培养物在液体培养基中重复体细胞胚胎发生。
Plant Cell Rep. 1992 Apr;11(3):122-5. doi: 10.1007/BF00232163.
4
Somatic embryogenesis and plant regeneration from leaflets of peanut, Arachis hypogaea.花生叶片体细胞胚胎发生和植株再生。
Plant Cell Rep. 1992 Mar;11(2):71-5. doi: 10.1007/BF00235256.
5
Plant regeneration via somatic embryogenesis in chick pea (Cicer arietinum L.).鹰嘴豆(Cicer arietinum L.)通过体细胞胚胎发生进行植株再生。
Plant Cell Rep. 1993 Sep;12(11):652-5. doi: 10.1007/BF00232818.
6
Culturing peanut (Arachis hypogaea L.) zygotic embryos for transformation via microprojectile bombardement.通过微弹轰击培养花生(落花生)合子胚进行转化。
Plant Cell Rep. 1993 Apr;12(6):316-9. doi: 10.1007/BF00237426.
7
Somatic embryogenesis from mature embryo-derived leaflets of peanut (Arachis Hypogaea L.).花生(落花生)成熟胚衍生叶片的体细胞胚胎发生。
Plant Cell Rep. 1994 Jul;13(10):578-81. doi: 10.1007/BF00234515.
8
Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.).通过体细胞胚胎发生再生羽扇豆(Cicer arietinum L.)。
Plant Cell Rep. 1994 May;13(8):468-72. doi: 10.1007/BF00231969.
9
Influence of photoperiod and medium formulation on peanut somatic embryogenesis.光周期和培养基配方对花生体细胞胚胎发生的影响。
Plant Cell Rep. 1994 Jan;13(3-4):159-63. doi: 10.1007/BF00239884.
10
High frequency somatic embryogenesis in peanut (Arachis hypogaea L.) using mature, dry seed.利用成熟、干燥的花生种子进行高频体细胞胚胎发生。
Plant Cell Rep. 1995 Jan;15(1-2):38-42. doi: 10.1007/BF01690250.