Department of Horticulture, University of Georgia, Coastal Plain Experiment Station, 31793, Tifton, GA, USA.
Plant Cell Rep. 1989 Apr;8(4):217-8. doi: 10.1007/BF00778536.
Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal medium. Shoots were excised from thick embryonic roots and rerooted on Murashige and Skoog medium containing half the normal concentration of inorganic salts. This technique should be useful for the production of interspecific hybrid plants from immatureArachis embryos.
花生(Arachis hypogaea L.)未成熟胚再生可以通过体细胞胚胎发生来实现。体细胞胚形成的最高频率发生在 B5 培养基中加入 0.5-1.0 mg/l picloram。只有在基本培养基上延长培养后,才能从体细胞胚中发育出芽和植株。从厚的胚根中切下芽,然后在含有正常无机盐浓度一半的 Murashige 和 Skoog 培养基上重新生根。该技术应该对从未成熟的花生胚胎生产种间杂种植物有用。