AFRC Institute of Food Research, Norwich Laboratory, NR4 7UA, Norwich Research Park, Colney, Norwich, UK.
Plant Cell Rep. 1992 Jun;11(5-6):270-3. doi: 10.1007/BF00235080.
Alkaloid production by several Agrobacterium rhizogenes-transformed root lines of Nicandra physaloides was studied. Early in the culture cycle all lines contained predominantly hygrine, which has previously been reported to be the major alkaloid in roots of this plant. A number of other hygrine-derived alkaloids were identified, and these generally increased in importance as the cultures matured. Some lines were found to differ quite markedly in the relative importance of the different hygrine-derived alkaloids. The possibility is raised that alkaloid biosynthesis in N. physaloides root cultures may be a good model system in which to study metabolite partitioning in branched metabolic pathways.
研究了几种发根农杆菌转化的 Nicandra physaloides 根系的生物碱产生。在培养周期的早期，所有系都主要含有 previously been reported to be the major alkaloid in roots of this plant 的腐胺，先前曾报道过腐胺是这种植物根中的主要生物碱。鉴定出了许多其他腐胺衍生的生物碱，随着培养物的成熟，这些生物碱的重要性通常会增加。发现一些系在不同腐胺衍生生物碱的相对重要性上差异非常明显。提出了这样一种可能性，即在 N. physaloides 根培养物中生物碱的生物合成可能是一个很好的模型系统，可以用来研究分支代谢途径中的代谢物分配。
