Section of Analytical Biochemistry, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, USA.
J Am Soc Mass Spectrom. 1996 Mar;7(3):293-7. doi: 10.1016/1044-0305(95)00703-2.
A new liquid chromatography-mass spectrometry technique is described that utilizes a particle beam interface to transport and deposit desolvated analyte molecules onto a target surface that is bombarded by a primary beam of massive multiply charged glycerol cluster ions to generate secondary ions for mass analysis. The massive cluster ion beam is generated by electrohydrodynamic emission from a solution of 1. 5-M ammonium acetate in glycerol. In the present instrumental configuration the massive cluster ion gun is placed above the target probe and the particle beam interface is connected through a side port of the mass spectrometer. The massive cluster ion beam and particle beam are intercepted by a target surface substituted for a conventional ion volume. The target surface is positioned such that it is ∼ 45 ° to the primary cluster ion beam, the particle beam, and the mass analyzer axis. This geometric orientation represents a compromise among the performances of these three elements.The feasibility of this liquid chromatography-particle beam on-line with massive cluster impact is demonstrated by flow injections of acetylcholine chloride and gramicidin S. Spectra generated from this preliminary study indicate promise for routine liquid chromatography-mass spectrometry of polar compounds by using a robust inlet and an effective generation of secondary ions without an added matrix.
描述了一种新的液相色谱-质谱技术，该技术利用颗粒束接口将脱溶剂的分析物分子输送和沉积到目标表面上，该目标表面受到大量多电荷甘油团簇离子的初级束的轰击，以产生用于质谱分析的次级离子。 大量团簇离子束是通过在甘油中的 1.5-M 乙酸铵溶液中电致流体发射产生的。 在当前的仪器配置中，将大量团簇离子枪放置在目标探头上方，并且通过质谱仪的侧端口连接颗粒束接口。 大量团簇离子束和颗粒束被目标表面截获，该目标表面替代了常规的离子体积。 目标表面的位置使其相对于主要团簇离子束，颗粒束和质量分析仪轴成约 45°。 这种几何取向是这三个元素性能之间的折衷。 通过氯化乙酰胆碱和短杆菌肽 S 的流动注射证明了这种液相色谱-颗粒束与大量团簇碰撞在线的可行性。 该初步研究产生的谱图表明，通过使用强大的进样器和有效的次级离子产生，无需添加基质，即可对极性化合物进行常规液相色谱-质谱分析。
