Mahoney J F, Cornett D S, Lee T D
Phrasor Scientific Inc., Duarte, California 91010.
Rapid Commun Mass Spectrom. 1994 May;8(5):403-6. doi: 10.1002/rcm.1290080513.
Massive-cluster impact is demonstrated to be an effective ionization technique for the mass analysis of proteins as large as 17 kDa. The design of the cluster source permits coupling to both magnetic-sector and quadrupole mass spectrometers. Mass spectra are characterized by the almost total absence of chemical background and a predominance of multiply charged ions formed from 100% glycerol matrix. The number of charge states produced by the technique is observed to range from +3 to +9 for chicken egg lysozyme (14,310 Da). The lower m/z values provided by higher charge states increase the effective mass range of analyses performed with conventional ionization by fast-atom bombardment or liquid secondary ion mass spectrometry.
大量簇撞击被证明是一种用于对高达17 kDa的蛋白质进行质量分析的有效电离技术。簇源的设计允许与磁扇形和四极质谱仪耦合。质谱的特点是几乎完全没有化学背景，并且由100%甘油基质形成的多电荷离子占主导。对于鸡蛋溶菌酶（14,310 Da），观察到该技术产生的电荷态数量范围为 +3至 +9。较高电荷态提供的较低m/z值增加了通过快速原子轰击或液体二次离子质谱进行常规电离分析的有效质量范围。
